Where do you measure the Bregma for rodent stereotaxic surgery?

نویسندگان

چکیده

The advent of the stereotaxic apparatus developed by Clarke and Horsley revolutionized neuroscience research, enabling precise 3D navigation along skull mediolateral, anteroposterior, dorsoventral axes. In rodents, Bregma is widely used as origin reference point for coordinates, but specific procedure its measurement varies among different laboratories. Notably, renowned brain atlas Paxinos Franklin lacks explicit instructions on determination. Recent studies have found discrepancies in landmark measurements. This review describes commonly atlases highlights limitations accurately measuring coordinates. addition, we propose alternative more reliable approaches to measure Bregma. It imperative address misconceptions about accuracy surgeries, it can significantly impact a substantial portion research.

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ژورنال

عنوان ژورنال: IBRO neuroscience reports

سال: 2023

ISSN: ['2667-2421']

DOI: https://doi.org/10.1016/j.ibneur.2023.07.003